In vitro Erythrocyte Membrane Stabilization Properties of
Solanum aethiopicum L. Fruit Extracts
Talha Bin Emran1,2*, Mir Muhammad Nasir Uddin3, Md. Atiar Rahman2, Md. Ismail Hossain1, Md.
Mohaiminul Islam1 and Md. Imtiazul Kabir4
1Department of Pharmacy, BGC Trust University Bangladesh, Chittagong-4000, Bangladesh. 2Department of Biochemistry and
Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh. 3Department of Pharmacy, University of Chittagong,
Chittagong-4331, Bangladesh. 4Department of Pharmacy, International Islamic University Chittagong, Chittagong-4203, Bangladesh.
ABSTRACT: This study aims at evaluating the membrane stabilization potential of Solanum aethiopicum L. fruits
extracts using an in vitro hemolytic assay. 2 mL of blood from healthy volunteers and patients with serologically
confirmed current dengue infection were freshly collected and used in the assays. Fresh garden eggs fruits at
three different maturity stages (immature, partly matured and matured) were cleaned with distilled water,
crushed and the juice was extracted with 10 mL of ice cold distilled water. Freshly prepared cold water extracts
of garden eggs fruits were used in the heat-induced and hypotonic-induced hemolytic assays. Extracts of garden
egg fruits of all three maturity levels showed a significant reduction in heat-induced hemolysis compared to
controls (P < 0.05). Garden egg fruits extracts of all three maturity levels showed more than 25% inhibition at a
concentration of 37.5μg/mL. Inhibition activity of different maturity levels was not significantly (P > 0.05)
different from one another. S. aethiopicum L. fruit extracts showed a significant inhibition of hemolysis in vitro.
KEYWORDS: Solanum aethiopicum L., erythrocyte membrane stabilization, dengue infection, hemolytic assays,
CITATION: Emran, T. B., Uddin, M. M. N., Rahman, M. A., Hossain, M. I., Islam, M. M. and Kabir, M. I. 2015. In vitro Erythrocyte
Membrane Stabilization Properties of Solanum aethiopicum L. Fruit Extracts. Biores Comm. 1(2), 111-115.