Betulin-3-caffeate and Amyrin from the Stem Bark of
Barringtonia acutangula (L)
Md. Enamul Haque1*, Zimam Mahmud1, A. K. M. Mahbub Hasan1, Roman Sardar1, Md. Lutful Kabir1 and Sheikh
1Department of Biochemistry and Molecular Biology, University of Dhaka. 2Department of Applied Chemistry and Chemical
Engineering, University of Dhaka.
ABSTRACT: Barringtonia acutangula (Indian Oak) is an important medicinal plant of Bangladesh and India for
its phytochemical and prognostic biomarkers in stem. This plant was selected based on its medicinal properties
including antiasthmatic, anticold, antimalerial, antidiarrehal, antiprotozoal, antidyesntry stimulating properties.
The coarsely powdered stem bark of B. acutangula was extracted with CHCl3. The concentrated extract was then
fractioned with flash column chromatography using petroleum ether, ethyl acetate and methanol. The column
fractions were further fractionated with gel permeating chromatography. Extensive preparative thin layer
chromatography resulted in the isolation of two compounds which were identified as betulin-3-caffeate and
amyrin. The structures of these compounds were determined by 1H NMR spectroscopy. This is the first report of
the isolation of these compounds from this plant stem bark.
KEYWORDS: Barringtonia acutangula, chromatography, betulin-3-caffeate, amyrin, 1H NMR.
CITATION: Haque, M. E., Mahmud, Z., Hasan, A. K. M. M., Sardar, R. and Haque, S. T. 2015. Betulin-3-caffeate and Amyrin from
the Stem Bark of Barringtonia acutangula (L). Biores Comm. 1(2), 121-123.