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Mouse homologues of hepatitis C virus human entry factors inhibit the entry of HCV pseudo-particles (HCVpp) into human hepatoma cells


Mohammod Johirul Islam1*, Mohammed Badrul Amin1, Mohammad Khaja Mafij Uddin1, Keisuke Hikosaka1, 2, Hidenao Noritake1, 3, Yi-Xin Wu1, Kazushi Aoto1 and Naoyuki Miura1

1Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192, Japan. 2Kitasato Research Center for Environmental Science, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0329, Japan. 3Division of Hepatology, Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192, Japan

ABSTRACT: Hepatitis C virus (HCV) is a growing public health concern worldwide. No vaccine preventing HCV has yet been developed due to the hindrance of research on suitable small animal models. Moreover, it was known that human CD81, claudin-1 (CLDN1), scavenger receptor type B class I (SCARB1) and occludin (OCLN) (collectively called CCSO) are important factors for HCV entry into human hepatocytes. Therefore, we previously generated transgenic mice expressing CCSO in their hepatocytes, but these mice do not confer susceptibility to HCVpp or HCV entry. Earlier study also showed mouse hepatocytes had strong restriction factor(s) that inhibit HCV entry. Here, we tried to find out the inhibitory factor(s), and found mouse homologues (Cd81, Ocln) themselves are inhibitory for HCV entry in human hepatoma cells when they express the mouse homologues on their cell surfaces. So, deleting these inhibitory factors can make mouse hepatocytes more susceptible to HCV infectivity in vivo. This in vitro study would thus be helpful in making an efficient mouse model for HCV infection.

KEYWORDS: Cd81, Ocln, NPC1L1, Huh7.5.1, NIH3T3

CITATION: Islam, M. J., Amin, M. B., Uddin, M. K. M., Hikosaka, K., Noritake, H., Wu, Y-X., Aoto, K., Miura, N. 2016. Mouse homologues of hepatitis C virus human entry factors inhibit the entry of HCV pseudo- particles (HCVpp) into human hepatoma cells. Biores Comm. 2(1), 128-133.

CORRESPONDENCE: Mohammod Johirul Islam, E-mail: johir@hama-med.ac.jp

 

 
 
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