Antioxidants and Total Phenolics of Ludwigia repens rubin Partially Improve the Hepatic Damage Induced by Carbon Tetra Chloride in Animal Model

Abstract

This research investigated the antioxidative and hepatoprotective properties of Ludwigia repens methanol extract. Antioxidative effects were measured by DPPH radical scavenging method and FeCl3 reducing power assay method. Total phenolic and total flavonoid were measured by established in vitro methods. Hepatoprotective potential was investigated by carbon tetrachloride (CCl₄) induced hepatotoxic model of Wistar Albino rat. Data were analyzed by using statistical software SPSS. The extract showed a dose-dependent antioxidative activity in all in vitro experiments. Free radical scavenging effect in terms of IC₅₀ value of the extract was found to be 75 µg/ml. Reducing power was gradually increased with the increase of concentration of the extract. Total phenolic content was 906.38 ± 15.95 mg gallic acid /g of dry weight of the extract and total flavonoid was 661.21 ± 2.20 mg of querecetine equivalent/g of L. repens extract. The hepatoprotective action of the plant extract at the dose of 300 mg/kg BW was evidenced by lowering of ALT, AST, ALP, bilirubin, total cholesterol, low-density lipoprotein (LDL) and triglycerides level. An increase level of protein synthesis and high-density lipoprotein (HDL) compared to CCl4 control group was also evident in treatment group. The study results suggested that oral intake of L. repens leaves extract may enhance the antioxidant defense status in the body and may reduce the risk of hepatic damage.