Development and validation of a novel customized medium for the marine microbial culture

Md. Morshedul Alam; Redwan Ahmed; Md. Ariful Amin; Mohammad Nazir Hossain

doi:10.3329/brc.v7i2.54374

Authors

Md. Morshedul Alam Department of Genetic Engineering & Biotechnology, Marine Biotechnology Lab, Bangladesh.Bangabandhu Sheikh Mujibur Rahman Maritime University, Mirpur-12, Dhaka-1216
Redwan Ahmed Department of Genetic Engineering & Biotechnology, Marine Biotechnology Lab, Bangladesh.Bangabandhu Sheikh Mujibur Rahman Maritime University, Mirpur-12, Dhaka-1216
Md. Ariful Amin Department of Genetic Engineering & Biotechnology, Marine Biotechnology Lab, Bangladesh.Bangabandhu Sheikh Mujibur Rahman Maritime University, Mirpur-12, Dhaka-1216
Mohammad Nazir Hossain Department of Genetic Engineering & Biotechnology, Marine Biotechnology Lab, Bangladesh.Bangabandhu Sheikh Mujibur Rahman Maritime University, Mirpur-12, Dhaka-1216

DOI:

https://doi.org/10.3329/brc.v7i2.54374

Keywords:

Marine microbes, marine microbial growth medium, novel customized medium

Abstract

Marine microbes are difficult to culture and hence it takes expensive measure to study. Mostly metagenomics are suggested for marine microbial characterization instead of culturing them in a suitable medium. In this study, a new approach has been developed to culture the marine microorganisms. We customized the LB agar and liquid medium by adjusting the pH and salt concentration at seawater level and then studied the microbial load, their growth rate by turbidity assay and also identified their morphology through gram staining. This study ensures the comfortable growth of marine microbes at the laboratory level, which would further help the marine biotechnologists and microbiologists to work smoothly with marine microbial samples.

References

Hunter, P. (2011), “Massing Life”, EMBO Reports, 11(7):511-514.

Suttle, C.A. (2005), “Viruses in the sea”, Nature 437:356-361.

Suttle, C.A. (2007), “Marine viruses: Major players in the global ecosystem”. National Review of Microbiology, 5(10):801-812.

Whiteman, W.B., Coleman D.C. and Wiebe, W.J. (1989), “Prokaryotes: The unseen majority”. Proceedings of the National Academy of Sciences, 95(12):6578-6583.

Hennes, K.P. and Suttle, C.A. (1995), “Direct counts of viruses in natural waters and laboratory cultures by epifluorescence microscopy”. Limnology and Oceanography, 40:1050-1055.