Resistance profiles of Azithromycin in Escherichia coli isolated from human urine

Resistance profiles of Azithromycin in Escherichia coli isolated from human urine

Shaikh Mirja Nurunnabi1*, Belal Ahmad.1, Joyonta Sutradhar1, Farjana Begum Topa1, Abu Noman1, Arifur Rahman1, Rashid Taqui Sakib1, Nishat Tasnim Nousin1, Shamim Mahmud1, Sadia Sinty Disha1, Ashik Imran1 and Mohammed Shahjahan2

1Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh. 2Rajshahi University, Dept. of Biochemistry and Molecular Biology, Rajshahi-6205, Bangladesh.

ABSTRACT: Bacterial drug resistance is now a main hindrance to use antibiotics in treating infectious diseases and global public health concern contributing to increased mortality in low-income countries. The plasmid mediated drug resistance which confers resistant to a number of antibiotics. Here, we studied the azithromycin susceptibility by paper disc plate technique and agar diffusion assay in bacterial strains collected from urine at the department of Microbiology Rajshahi Medical College Hospital, Rajshahi. We found that azithromycin at certain concentration is resistant to Escherichia coli (E. coli) but not Klebsiella, Proteus, Streptococcus and Pseudomonus strains. Furthermore, E. coli was also resistant to number of commonly used antibiotics like Ampicillin, Amoxicillin, Tetracycline, Doxycycline, Ciprofloxacin, Cephradine, PenicillinG and Chloramphenicol. In present study plasmid DNA was isolated from azithromycin resistant E .coli. Together, our primary results suggest that E. coli is multidrug resistant due to possibly the presence of approximately 4kb in size of resistant plasmid in Rajshahi area.

KEYWORDS: Resistance profile, Azithromycin, E.coli, Urine sample, Resistant plasmid, Rajshahi area.

CORRESPONDENCE: Shaikh Mirja Nurunnabi