Isolation of muitidrug resistant Listeria monocytogenes from processed Rhynchophorus phoenicis Larvae

Abstract

Listeria monocytogenes was isolated from two hundred (200) samples of roasted Rhynchophorus phoenicis larvae samples by pour plate methods on Listeria selective agar. A total of 67 Listeria spp. were isolated of which 28 (41.79%) were identified biochemically and serologically as L. monocytogenes. All L. monocytogenes successfully amplified the hlyA gene virulent markers. Antibitoics susceptibility pattern of L. monocytogenes showed that all isolates were resistant to cloxacillin, augumentin, ceftrizone, and amoxicillin. Other isolates showed varying antibiotics resistance pattern with 42.86% resistance to tetracycline, 21.43% for gentamycin, 35.71% for streptomycin, 50.00% for ciprofloxacin, and 46.43% for erythromycin. The study revealed that 100% of the isolates were resistant to four antibiotics while 71.43% and 57.14% of the isolates were resistant to five and more than five antibiotics.