Significant Association of ADAM33 V4C>G Polymorphisms with Asthma in a North Indian Population

Abstract

ADAM33 is zinc-dependent metalloproteases comprised of seven domains and has range of single nucleotide polymorphisms. These polymorphisms were found to be genetically associated in bronchial hyperresponsiveness that accounts its involvement in asthma. A case-control study was conducted with 483 healthy controls and 481 asthma patients in the present study. DNA samples were extracted from blood and the genotyping was done using PCR-RFLP method. Statistical analysis revealed that ADAM33 V4C>G polymorphism show highly significant association towards asthma with significant OR=1.40, p=0.017 in CG genotype and two fold risk in mutant GG genotype (p=0.000). Furthermore, mutant allele G was also highly significant towards the disease with OR=1.39 and p=0.000. However, ADAM33 F+1G>A, rs511898 gene polymorphism was significantly associated with asthma in only male and female phenotypes. This study concludes that the ADAM33 gene polymorphism plays an important role in the pathogenesis of asthma in a North Indian population.