LISTERIA MONOCYTOGENES IN READY-TO-EAT CHICKEN PRODUCTS, THEIR ANTIBIOTIC RESISTANCE AND VIRULENCE GENES
DOI:
https://doi.org/10.3329/brc.v9i1.63595Keywords:
Ready-to-eat meat, virulence, antibiotic resistance, BangladeshAbstract
Ready-to-eat (RTE) meat products are food items that are extremely popular worldwide. Albeit documented with the alarming prevalence of pathogenic multidrug-resistant Listeria species in these food items worldwide, studies from the perspective of Bangladesh are absent. This study was devised with the aim to detect pathogenic Listeria monocytogenes in some of the most popular RTE meat products in Dhaka, Bangladesh. Thirty-nine such samples were investigated using biochemical tests and the Polymerase Chain Reaction (PCR) analysis. Following confirmatory tests and detection of Listeria species, the isolates were subjected to the Kirby-Bauer disc diffusion test to investigate their antibiotic-susceptibility patterns against some of the most commonly used antibiotics to treat listeriosis – the infection caused by Listeria. Five Listeria species were detected using biochemical and PCR tests. This constituted an overall prevalence rate of 12.8% (n = 39). Four out of the five Listeria species were concluded to be L. monocytogenes, while the remaining one was an L. innocua isolate. PCR analyses revealed all four of the L. monocytogenes isolates to have the virulence genes hlyA, plcB and actA, although no polymorphism was observed for these genes. Despite the presence of these pathogenic genes, antibiotic susceptibility tests showed promising results as some of the most commonly prescribed drugs against listeriosis were highly effective against the isolates. Even though the isolates showed little to no antibiotic resistance against the antibiotics used in the study, the presence of three virulence genes in all four L. monocytogenes isolates is still a cause for concern. Even more so, the prevalence of these pathogenic strains in a food item that is increasing in popularity in a country like Bangladesh, where awareness and surveillance against listeriosis is limited, can have dire consequences should an outbreak occurs.
References
Bai J, Shi X, Nagaraja T. G (2010) A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7. Journal of Microbiological Methods 82:85–89. https://doi.org/10.1016/j.mimet.2010.05.003
Bajkó Z, Bălaşa R, Maier S, Moţăţăianu A, Treabă A, Macarie I, Gârbovan C, Chiriac C (2013) Listeria monocytogenes meningoencephalitis mimicking stroke in a patient with chronic lymphocytic leukemia. Neurology and Therapy 2:63–70. https://doi.org/10.1007/s40120-013-0009-y
Borucki MK, Gay CC, Reynolds J, McElwain KL, Kim SH, Call DR, Knowles DP (2005) Genetic diversity of Listeria monocytogenes strains from a high-prevalence dairy farm. Applied and Environmental Microbiology 71:5893–5899. https://doi.org/10.1128/AEM.71.10.5893-5899.2005
Bustamante F, Maury-Sintjago E, Cerda Leal F, Acuña S, Aguirre J, Troncoso M, Figueroa G, Parra-Flores J (2020) Presence of listeria monocytogenes in ready-to-eat artisanal chilean foods. Microorganisms 8:1–18. https://doi.org/10.3390/microorganisms8111669
Carpentier B, Cerf O (2011) Persistence of Listeria monocytogenes in food industry equipment and premises. International Journal of Food Microbiology 145:1–8. https://doi.org/ 10.1016/j.ijfoodmicro.2011.01.005
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
Gebretsadik S, Kassa T, Alemayehu H, Huruy K, Kebede N (2011) Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal origin in Addis Ababa, Ethiopia. Journal of Infection and Public Health 4:22–29. https://doi.org/10.1016/j.jiph.2010.10.002
Gil M I, Allende A, López Gálvez F, Selma MV (2009) Hay alternativas al cloro como higienizante para productos de IV Gama. Revista Horticultura. Mayo. http://www.horticom.com/revistasonline/extras/extra09/38_45.pdf. Accessed 3 May 2021
Hudzicki J (2012) Kirby-Bauer Disk Diffusion Susceptibility Test Protocol Author Information. American Society For Microbiology, December 2009, 1–13. https://www.asm.org/Protocols/Kirby-Bauer-Disk-Diffusion-Susceptibility-Test-Pro. Accessed 1 May, 2021
Islam MS, Husna AA, Islam MA, Khatun M M (2016) Prevalence of Listeria monocytogenes in Beef, Chevon and Chicken in Bangladesh. American Journal of Food Science and Health 2:39–44. http://www.aiscience.org/journal/ajfshhttp://creativecommons.org/licenses/by/4.0/
Issa ZM, Yen LH, Radu S (2011) Antibiogram Profiles of Listeria monocytogenes isolated from foods. 2011 2nd International Conference on Biotechnology and Food Science 7:133–137. http://www.ipcbee.com/vol7/31-ICBFS2011S10043.pdf. Accessed 10 May, 2021
Jaradat ZW, Schutze GE, Bhunia AK (2002) Genetic homogeneity among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes. International Journal of Food Microbiology 76:1–10. https://doi.org/10.1016/S0168-1605(02)00050-8
Jiang H, Dong H, Zhang G, Yu B, Chapman LR, Fields MW (2006) Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Applied and Environmental Microbiology, 72:3832–3845. https://doi.org/10.1128/AEM.02869-05
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120. https://doi.org/10.1007/BF01731581
Kumar BNV (2016) Molecular characterization and antibiogram of Listeria monocytogenes isolated from chicken and mutton of retail markets. Journal of Microbiology and Infectious Diseases 6:65–68. https://doi.org/10.5799/ahinjs.02.2016.02.0218
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33:1870–1874. https://doi.org/10.1093/molbev/msw054
Larsen, MH, Kallipolitis BH, Christiansen JK, Olsen JE, Ingmer H (2006) The response regulator ResD modulates virulence gene expression in response to carbohydrates in Listeria monocytogenes. Molecular Microbiology, 61:1622–1635. https://doi.org/10.1111/j.1365-2958.2006.05328.x
Listeria monocytogenes (Murray et al.) Pirie ATCC ® 19117TM. (n.d.).
https://www.atcc.org/products/all/19117.aspx#generalinformation. Accessed April 29, 2020
Manjur MSE, Siddique S, Ahmed S (2018) Multi-drug resistant pathogenic Listeria monocytogenes in surface water and soil samples of Dhaka city. Bangladesh Journal of Microbiology 33:39–42.
https://doi.org/10.3329/bjm.v33i1.39602
Martínez-Martínez L, Joyanes P, Suárez AI, Perea EJ (2001) Activities of gemifloxacin and five other antimicrobial agents against Listeria monocytogenes and coryneform bacteria isolated from clinical samples. Antimicrobial Agents and Chemotherapy 45:2390-2392. https://doi.org/10.1128/AAC.45.8.2390-2392.2001
Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RV (1999) Food-related illness and death in the United States. Emerging Infectious Diseases 5:607–625. https://doi.org/10.3201/eid0505.990502
Mehlhorn, H (2015) Food-Borne Disease Burden Epidemiology Reference Group. Encyclopedia of Parasitology, 1–1. https://doi.org/10.1007/978-3-642-27769-6_3884-1
Olaniran AO, Nzimande SBT, Mkize NG (2015) Antimicrobial resistance and virulence signatures of Listeria and Aeromonas species recovered from treated wastewater effluent and receiving surface water in Durban, South Africa. BMC Microbiology 15:1–10. https://doi.org/10.1186/s12866-015-0570-x
Poyart-Salmeron C, Carlier C, Trieu-Cuot P, Courtieu AL, Courvalin P (1990) Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet (London, England) 335:1422–1426. https://doi.org/10.1016/0140-6736(90)91447-i
Prevention, C. for D. C. and. (2013). CDC. (2013) Multistate outbreak of listeriosis linked to whole cantaloupes from Jensen Farms, Colorado. https://www.cdc.gov/listeria/outbreaks/cantaloupes-jensen-farms/index.html. Accessed 5 May 2021
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, Jones, J L, Griffin PM (2011) Foodborne illness acquired in the United States-Major pathogens. Emerging Infectious Diseases 17:7–15. https://doi.org/10.3201/eid1701.P11101
Scotter SL, Langton S, Lombard B, Schulten S, Nagelkerke N, In’t Veld PH, Rollier P, Lahellec C (2001) Validation of ISO method 11290 part 1--detection of Listeria monocytogenes in foods. International Journal of Food Microbiology 64:295–306. https://doi.org/10.1016/s0168-1605(00)00462-1
Shamloo E, Hosseini H, Moghadam AZ, Larsen HM, Haslberger A, Alebouyeh M (2019) Importance of Listeria monocytogenes in food safety: A review of its prevalence, detection, and antibiotic resistance. In Iranian Journal of Veterinary Research 20:241–254. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983307/
Shourav AH, Hasan M, Ahmed S (2020) Antibiotic susceptibility pattern of Listeria spp. isolated from cattle farm environment in Bangladesh. Journal of Agriculture and Food Research 2:100082. https://doi.org/10.1016/j.jafr.2020.100082
SR0141, Listeria Selective Enrichment Supplement | Oxoid - Product Detail. (n.d.). http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=SR0141&cat=&c=UK&lang=EN. Accessed August 21, 2020
Todd ECD, Notermans S (2011) Surveillance of listeriosis and its causative pathogen, Listeria monocytogenes. Food Control 22:1484–1490. https://doi.org/10.1016/j.foodcont.2010.07.021
Uyttendaele M, De Troy P, Debevere J (1999) Incidence of Listeria monocytogenes in different types of meat products on the Belgian retail market. International Journal of Food Microbiology 53:75–80. https://doi.org/10.1016/S0168-1605(99)00155-5
Vines A, and Swaminathan B (1998) Identification and characterization of nucleotide sequence differences in three virulence-associated genes of Listeria monocytogenes strains representing clinically important serotypes. Current Microbiology 36:309–318. https://doi.org/ 10.1007/s002849900315
Vital Signs: Listeria Illnesses, Deaths, and Outbreaks — United States, 2009–2011. (n.d.). https://www.cdc.gov/mmwr/preview/mmwrhtml/mm6222a4.htm. Accessed April 29, 2021
Walker SJ, Archer P, Banks JG (1990) Growth of Listeria monocytogenes at refrigeration temperatures. Journal of Applied Bacteriology 68:157–162. https://doi.org/ 10.1111/j.1365-2672.1990.tb02561.x
Wallace C., Sperber W., Mortimore S. Food Safety for the 21st Century, Managing HACCP and Food Safety Throughout the Global Supply Chain. 2nd ed. Wiley; Minnesota, MN, USA: 2018. p. 396.
Wiedmann M, Bruce JL, Keating C, Johnson AE, McDonough PL, Batt CA (1997) Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infection and Immunity 65:2707–2716. https://doi.org/10.1128/iai.65.7.2707-2716.1997
Zhu Q, Gooneratne R, Hussain MA (2017) Listeria monocytogenes in fresh produce: outbreaks, prevalence and contamination levels. Foods 6:21. https://doi.org/10.3390/foods6030021
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