GENOMIC ANALYSIS TO ELUCIDATE THE ANTIBIOTIC RESISTANCE MECHANISM OF EXTREMELY DRUG-RESISTANT PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM BANGLADESH

Fatimah Az Zahra; Ishrat Jabeen; Mohammed Jafar Uddin; Nazmun Nahar; Sohidul Islam; Sabbir R. Shuvo

doi:10.3329/brc.v9i1.63601

Authors

Fatimah Az Zahra Department of Biochemistry & Microbiology, North South University, Dhaka 1229, Bangladesh
Ishrat Jabeen Department of Biochemistry & Microbiology, North South University, Dhaka 1229, Bangladesh
Mohammed Jafar Uddin Department of Biochemistry & Microbiology, North South University, Dhaka 1229, Bangladesh
Nazmun Nahar Department of Biochemistry & Microbiology, North South University, Dhaka 1229, Bangladesh
Sohidul Islam Department of Biochemistry & Microbiology, North South University, Dhaka 1229, Bangladesh
Sabbir R. Shuvo Department of Biochemistry & Microbiology, North South University, Dhaka 1229, Bangladesh

DOI:

https://doi.org/10.3329/brc.v9i1.63601

Keywords:

Pseudomonas aeruginosa, whole-genome sequencing, multidrug-resistant, Bangladesh

Abstract

Multidrug-resistant P. aeruginosa has potential to cause nosocomial infections. In this study, whole-genome sequencing was performed of two extremely drug-resistant novel strains SRS1 and SRS4 isolated from Bangladesh. The size of draft genome of SRS1 is 6.8 Mbp, and 7.0 Mbp for SRS4. In silico analysis predicted that the genome of SRS1 has 82 and SRS4 has 75 antibiotic-resistant genes (ARGs). Antibiogram results revealed that both SRS1 and SRS4 were resistant to multiple members of the antibiotic groups of β−lactam, quinolones, and aminoglycosides families. In addition, the genomes of both SRS1 and SRS4 were predicted to have multiple mobile elements like prophages and plasmids. Comparative genome analysis with wildtype PAO1 and another drug-resistant P. aeruginosa strain JNQH-PA57 revealed that SRS1 and SRS4 contain more antibiotic resistance genes like AAC (6´)-II, ANT (2´´)-Ia, ANT (3´´)-IIa, OXA-395, PME-1, qacE∆1, tet(A), tet(D), VEB-9 than PAO1 and JNQH-PA57. This study shows the importance of the genomic study to understand the distribution of ARGs in Bangladeshi P. aeruginosa strains to demonstrate the mechanisms responsible for multi drug resistance.

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