IN VITRO CONTROL OF PATHOGENIC FUNGI ASSOCIATED WITH SELECTED BRRI RICE VARIETIES

Sarowar Hosen; Shamim Shamsi; Swastika  Chakravorty

doi:10.3329/brc.v10i1.70668

Authors

Sarowar Hosen Department of Botany, University of Dhaka, Dhaka‐1000, Bangladesh
Shamim Shamsi Department of Botany, University of Dhaka, Dhaka‐1000, Bangladesh
Swastika Chakravorty Department of Botany, University of Dhaka, Dhaka‐1000, Bangladesh

DOI:

https://doi.org/10.3329/brc.v10i1.70668

Keywords:

Pathogenic fungi, In vitro, Fungicides, Plant extracts, BRRI rice varieties

Abstract

The present study evaluated the efficacy of five chemical fungicides, viz., Capvit 50 WP, Cynil 72 WP, Kochi 80 WDG, Sinozim 50 WP and Ridomil Gold MZ 68 WG along with five plant extracts, specifically Azadirachta indica A. Juss., Adhatoda vasica Nees., Psidium guajava L., Cassia alata L. and Citrus limon on the radial mycelial growth of two pathogenic fungi, Curvularia lunata and Drechslera oryzae associated with newly released BRRI rice varieties at varying concentrations. Capvit 50 WP exhibited complete inhibition of C. lunata at 300, 400 and 500 ppm concentrations as well as D. oryzae at 200, 300, 400 and 500 ppm concentrations leading the selected fungicides, followed by Cynil 72 WP and Ridomil Gold MZ 68 WG. Out of the five plant extracts, P. guajava demonstrated maximum radial growth inhibition (72.2%) against C. lunata at 20% concentration followed by A. indica. (56%), A. vasica. (52.3%), C. alata (24%) and C. limon (18.1%). Capvit 50 WP and Cynil 72 WP, among the fungicides, are potential in vivo controls for the tested pathogens associated with rice varieties. Additionally, out of the five plant extracts, Psidium guajava L. can be effective against C. lunata, while Azadirachta indica A. Juss. is a promising botanical fungicide against D. oryzae.

References

Misra, J.K., Gergon, E.B. and Mew, T.W. (1995), Storage fungi and seed health of rice: a study in the Philippines, Mycopathologia, 131(1), 13-24.

Fakir, G.A., Hossain, I, Ahmed, M.U., Anam, M.K., Alam, M.N. and Rahtnan, M. (2003), Effect of ash, chalk powder and neem leaf on the quality of borne rice seed stored in gunny bag, motka, plastic drum and tin, Proceedings of review and planning meeting of the Rice seed health improvement Sub-project held at BRRI, Gazipur, Bangladesh, pp. 1-37.

Fakir, G.A., Hossain, I., Ahmad, M.U., Asad-ud-Doullah, M. and Alam, M. (2002), Quality of farmer’s Boro and T. Aman rice seeds collected before sowing from Bogra, Rajshahi and Rangpur Districts of Bangladesh. A paper presented in the Review and planning meeting of the rice seed health improvement (PETRRA) project, held on 21-22 April at BRRI, Gazipur, Bangladesh.

Bhandari, H., Mohanty, S and Hossain, M. (2011), Hybrid rice in Bangladesh: Current status and future project. In Proceeding of the 7th ASAE conference 2011, Hanoi, Vietnam, pp. 13-15.

Miah, S.A., Shahjahan, A.K.M., Hossain, M.A. and Sharma, N.R. (1985), Survey of rice disease in Bangladesh, Tropical Pest Management, 31(3), 208-213.

Chowdhury, P., Shamsi, S. and Bashar, M.A. (2018), Mycoflora associated with diseased rice grains and their pathogenic potentiality. Abstract presentation in Annual Botanical Conference 2018, 16th March, 2019, Cox’s Bazar, Bangladesh.

Sultana, T., Shamsi, S. and Bashar, M.A. (2018), Prevalence of fungi with seeds of twenty BRRI released rice varieties and seed quality analysis. Journal of Asiatic Society Bangladesh, Sci., 44(1), 79-89.

ISTA, (1996), International Rules of Seed Testing Association, In. Proc. Int. Seed Test. Assoc., pp. 19-41.

CAB, (1968), Plant Pathologist’s Pocket Book, 1st edition, The Commonwealth Mycological Institute, England, pp. 267.

Thom, C. and Raper, K.B. (1945), A manual of the Aspergilli. Williams and Wilkins, Baltimore, M.D. USA, pp. 373.

Benoit, M.A. and Mathur, S.B. (1970), Identification of species Curvularia on rice seed. Proc. Inst. Seed Test. Assoc., 35(1), 1-23.

Ellis, M.B. (1971), Dematiaceous Hyphomycetes, The Commonwealth Mycological Institute, England, pp. 608.

Ellis, M.B. (1976), More Dematiaceous Hyphomycetes, The Commonwealth Mycological Institute, England, pp. 507.

Barnett, H. L. and Hunter, B.B. (2000), Illustrated Genera of Imperfect Fungi, 4th edn., Burges Pub., Co. Minneapolis, pp. 185.

Reddy, K.S. and Subbayya, J. (1989), Evaluation of different methods of inoculation for screening rice cultivars for sheath rot resistance. Oryza, 26, 416-417.

Shamsi, S., Chakravorty, S., and Hosen, S. (2023), Prevalence of seed-borne fungi associated with selected rice varieties of BRRI, Journal of Biodiversity Conservation and Bioresource Management, 9(1), 61–68.

Chowdhury, P., Bashar, M.A. and Shamsi, S. (2015), In vitro evaluation of fungicides and plant extracts against pathogenic fungi of two rice varieties, Bangladesh J. Bot., 24(2), 251-259.

Nahar, M.N. and Shamsi, S. (2020), In vitro screening of fungicides and plant extracts against six pathogenic fungi isolated from cotton (Gossypium arboreum L.) seed, Bangladesh J. Bot., 49(2), 197-204.