BIOCHEMICAL ANALYSIS OF LEONURUS SIBIRICUS AND PREDICTION OF ITS BIOACTIVE COMPOUND-PROTEIN INTERACTIONS IN HOMO SAPIENS

Abdirizak Abdirahman Osman; Ahmed Abdukadir Abdi Omar; Mst. Ananya Khatun; Md. Ramjan Sheikh; Md. Suzaul Alam; Md. Maruf Uz Zaman; Md. Tanvir Abedin; Md. Abu Sayed

doi:10.3329/brc.v10i1.70669

Authors

Abdirizak Abdirahman Osman Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh
Ahmed Abdukadir Abdi Omar Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh
Mst. Ananya Khatun Department of Horticulture, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh
Md. Ramjan Sheikh Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh
Md. Suzaul Alam Department of Biochemistry and Molecular Biology, Prime Institute of Science and Medical Technology, Rangpur, Bangladesh
Md. Maruf Uz Zaman Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh
Md. Tanvir Abedin Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh
Md. Abu Sayed Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh

DOI:

https://doi.org/10.3329/brc.v10i1.70669

Keywords:

Functional annotation, honeyweed, proximate composition, quercetin, rutin

Abstract

This study was conducted to investigate the proximate composition of L. sibiricus, an herbaceous medicinal plant, and explore the potential effects of its bioactive compounds, rutin and quercetin. Samples were collected from Dinajpur and Nilphamari to assess proximate composition. Proximate composition analysis revealed variations of ash, starch, fat, protein, non-reducing sugar (sucrose), invert (total) sugar, and reducing sugar contents of L. sibiricus. These findings indicate that proximate compositions and mineral contents varied depending on the growing place, soil and environmental factors. Furthermore, bioinformatics tools were utilized to investigate the effects of bioactive compounds rutin and quercetin of L. sibiricus in Homo sapiens. In this study, both rutin and quercetin interacted with 10 proteins, separately. Each individual protein interacted with another 10 proteins and found 100 proteins for rutin and 99 proteins for quercetin. However, 199 proteins were used to blast search in NCBI database and blasted results found only 177 proteins. Venn diagram analysis showed only four (4) common proteins interacted with rutin and quercetin. Functional annotation analysis revealed both positive and negative regulations of rutin and quercetin for biological process, molecular functions and cellular component in human health. Interestingly, KEGG pathway, compound and drug analysis exhibited that some potential drugs have already been discovered by rutin and quercetin that are mostly used for vascular protectant, ophthalmic, liver function improving agent and antimicrobial activity. A number of genes or enzymes involved in rutin and quercetin biosynthesis pathway retrieved in KEGG database. This study revealed that genetic engineering might enhances the biosynthesis of rutin and quercetin in honeyweed that could be utilized for production of valuable drug in industry for controlling different human and animal diseases in Bangladesh.

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