Evaluation of the Life Cycle of Aedes albopictus and Its Control Strategy Using Deltamethrin and Lambda-Cyhalothrin as Larvicides

Mohammad Bakhtear  Uddin; Ismat Zaman  Urboshi; Tahsin  Tamanna; Omar  Faruque; Atia Shanjida  Shormi; Hafisha Khatun  Anee; Khandaker Ashfaqul  Muid

doi:10.3329/brc.v11i2.82644

Authors

Mohammad Bakhtear Uddin Branch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Bangladesh
Ismat Zaman Urboshi Branch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Bangladesh
Tahsin Tamanna Branch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Bangladesh
Omar Faruque Branch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Bangladesh
Atia Shanjida Shormi Branch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Bangladesh
Hafisha Khatun Anee Branch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Bangladesh
Khandaker Ashfaqul Muid Branch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Bangladesh

DOI:

https://doi.org/10.3329/brc.v11i2.82644

Keywords:

Aedes albopictus, lambda-cyhalothrin, deltamethrin, resistance, cytogenotoxicity

Abstract

Dengue fever, transmitted by Aedes aegypti and Aedes albopictus, has seen a global increase, with A. albopictus particularly prevalent in Dhaka, Bangladesh, during the study period, August 2023 to July 2024. This study focused on controlling dengue at the vector level, specifically targeting A. albopictus, which was identified in both larval and adult stages through morphological analysis. Results showed that A. albopictus larvae took about three times longer to develop into adults during winter than in summer. The effectiveness of two pyrethroid insecticides, lambda-cyhalothrin and deltamethrin, was assessed by determining lethal concentrations (LC₅₀ and LC₉₀), emergence inhibition (IE₅₀ and IE₉₀), and resistance ratios (RR) for late 3rd and early 4th instar larvae. Current LC₅₀ and LC₉₀ values for lambda-cyhalothrin were 0.29 ppm and 0.47 ppm, while deltamethrin values were 0.24 ppm and 0.69 ppm, showing a significant rise from previous levels and indicating increased resistance (p<0.05). Cytogenotoxicity tests of the determined doses revealed minimal DNA damage in human blood nucleoids and insignificant impact on mammalian cell viability. These findings suggest that lambda-cyhalothrin and deltamethrin remain effective against A. albopictus larvae without substantial genomic damage on non-target organisms; however, further study is needed to evaluate long-term effects and potential resistance mechanisms.

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