Molecular Identification of Six New Benthic Fauna Records in the Andharmanik River, Bangladesh

Rifat Ferdous  Ananya; Sujan Kumar  Datta; Mysha  Mahjabin; Md Sagir  Ahmed

doi:10.3329/brc.v11i2.82649

Authors

Rifat Ferdous Ananya Department of Zoology, University of Dhaka, Dhaka 1000, Bangladesh
Sujan Kumar Datta Department of Zoology, University of Dhaka, Dhaka 1000, Bangladesh
Mysha Mahjabin Department of Zoology, University of Dhaka, Dhaka 1000, Bangladesh
Md Sagir Ahmed Department of Zoology, University of Dhaka, Dhaka 1000, Bangladesh

DOI:

https://doi.org/10.3329/brc.v11i2.82649

Keywords:

Andharmanik River, Benthos, Nemertean, New records, Polychaete, DNA Barcoding

Abstract

DNA barcoding has proven to be an effective alternative to traditional morphological methods for species identification. In this study, we employed a molecular approach to identify benthic fauna collected from the Andharmanik River estuary in Bangladesh. Mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal RNA (16S rRNA) genes were used as genetic markers for species delineation. A total of six benthic species—Cerebratulus lacteus, Maculaura magna, Maculaura cerebrosa, Perinereis cultrifera, Paralacydonia weberi, and Phyllodoce medipapillata—were reported for the first time in Bangladesh in this study. The mean interspecific genetic divergence, calculated using the Kimura 2-Parameter (K2P) model, was 39.72 ± 0.07% for 16S rRNA and 29.72 ± 0.03% for COI, indicating sufficient resolution for accurate species discrimination. Phylogenetic relationships were inferred using the Maximum Likelihood method, and individuals belonging to the same species consistently formed species-specific clades. This study presents the first DNA barcoding-based identification of polychaete and nemertean species in Bangladesh.

References

Edgar, R. C. (2004). MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC bioinformatics, 5, 1-19.

Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294–299.

Hebert, P. D. N., Cywinska, A., Ball, S. L., & deWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1512), 313–321. https://doi.org/10.1098/rspb.2002.2218

Hiebert, T. C., & Maslakova, S. (2015). Integrative Taxonomy of the Micrura alaskensis Coe, 1901 Species Complex (Nemertea: Heteronemertea), with Descriptions of a New Genus Maculaura gen. nov. and Four New Species from the NE Pacific. Zoological Science, 32(6), 615–637. https://doi.org/10.2108/zs150011

Horst, R. (1923). On three remarkable Annelida Polychaeta. Zoologische Mededelingen, 7(12), 221–224

Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16, 111-120.

Kinberg, J.G.H. (1865). Annulata nova. Öfversigt af Königlich Vetenskapsakademiens förhandlingar, Stockholm. 22(2): 167-179

Leidy, P, (1851). Helminthological Contributions No. 3. Proceedings of the Academy of Natural Sciences of Philadelphia,. 5: 239–244

Maturana, C. S., Moreno, R. A., Labra, F. A., González-Wevar, C. A., Rozbaczylo, N., Carrasco, F. D., & Poulin, E. (2011). DNA barcoding of marine polychaetes species of southern Patagonian fjords. Revista de Biología Marina y Oceanografía, 46(1), 35–42. https://doi.org/10.4067/S0718-19572011000100005

Mohsin, A. B. M., Yeasmin, F., Galib, S. M., Alam, B., & Haque, S. M. (2014). Fish Fauna of the Andharmanik River in Patuakhali, Bangladesh.

Moore, J. Percy. (1909). Polychaetous annelids from Monterey Bay and San Diego, California. Proceedings of the Academy of Natural Sciences of Philadelphia. 61: 235-295.

Mustafa, T., Alam, S. M. I., Jamal, M. S., Johora, F. T., Likhon, M. A. K., Hoq, T., & Naser, M. N. (2015). Abundance of benthic fauna in winter and summer seasons at three water bodies of Dhaka, Bangladesh. Bangladesh Journal of Zoology, 41(1), 79–86. https://doi.org/10.3329/bjz.v41i1.23301

Palumbi, S. R. (1996). What can molecular genetics contribute to marine biogeography? An urchin’s tale. Journal of Experimental Marine Biology and Ecology, 203(1), 75–92. https://doi.org/10.1016/0022-0981(96)02571-3

Rouse, G. W., & Pleijel, F. (2001). Polychaetes. Oxford University Press.

Roy, D., Didar, N. B., Sarker, S., Rahman Khan, Md. A., & Latifa, G. A. (2022). Appraisal of different attributes of fish community in Andharmanik River of coastal Bangladesh and socio-economic conditions of fishermen. Heliyon, 8(7), e09825. https://doi.org/10.1016/j.heliyon.2022.e09825

Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: molecular evolutionary genetics analysis version 11. Molecular biology and evolution, 38(7), 3022-3027.