Human Amniotic Membrane and Curcuma Longa–Derived Gel for the Treatment of Burn Injuries in A Rat Model

Hafizur Rahman; Ayesha  Siddika; Polash Chandra  Karmakar; Tusher-  Al-Arafat; Liakat  Hossain; Hasib  Adnan; Farha Matin  Juliana; Naznin  Akhtar; S. M.  Asaduzzaman

doi:10.3329/brc.v12i1.86763

Authors

Hafizur Rahman Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh
Ayesha Siddika Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment (AERE), Savar, Dhaka 1349, Bangladesh
Polash Chandra Karmakar Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment (AERE), Savar, Dhaka 1349, Bangladesh
Tusher- Al-Arafat Department of Microbiology, Jashore University of Science and Technology, Jashore 7408, Bangladesh
Liakat Hossain Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment (AERE), Savar, Dhaka 1349, Bangladesh
Hasib Adnan Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment (AERE), Savar, Dhaka 1349, Bangladesh
Farha Matin Juliana Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh
Naznin Akhtar Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment (AERE), Savar, Dhaka 1349, Bangladesh
S. M. Asaduzzaman Institute of Tissue Banking and Biomaterial Research, Atomic Energy Research Establishment (AERE), Savar, Dhaka 1349, Bangladesh

DOI:

https://doi.org/10.3329/brc.v12i1.86763

Keywords:

Human Amniotic Membrane, Curcuma longa, Burn wounds, Wound contraction, Epidermal regeneration

Abstract

Purpose: Burn injuries are a serious medical issue worldwide. In this study, Curcuma longa (CUR) loaded human amniotic membrane (HAM) gel was formulated to find an effective treatment for burn wounds with a faster healing rate and cost-effectiveness. Methodology: Physicochemical evaluation of the gel formulations was carried out through visual examination, assessing factors such as consistency, homogeneity, pH, FTIR, NMR analysis, and brine shrimp lethality test. To determine the burn wound healing efficacy, 20 female Wistar rats with 150-180g body weight were randomly divided into five groups: negative control, HAM, CUR, HAM+CUR and positive control. Burns were created and then treated with respective gels. Major findings: All gel formulations were essentially homogeneous and had good consistency with a pH between 6.68 and 6.80. FTIR and NMR studies supported the presence of collagen and skin irritation study displayed no edema or erythema after 7 days of topical application. After 16 days of treatment, the HAM+CUR group showed superiority (94.31±2.24 %) in burn wound healing, which was statistically the most significant compared to the control group (P<0.001). In addition, it takes only 19.67±1.52 days for the HAM+CUR group for re-epithelialization, while HAM, CUR, positive and the negative control group required 25.33±1.53, 24.67±2.08, 22.0±2, and 31.67 ±1.15 days for re-epithelialization, respectively. Histological evaluation revealed that HAM+CUR treated rat groups had significant epidermal regeneration, granulation tissue formation and collagen deposition. Conclusions: Therefore, HAM+CUR gel demonstrates significant potential therapeutic agent for treating burn wounds.

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