Antagonistic potentiality of six wheat rhizosphere fungi against leaf blight pathogen Bipolaris sorokiniana (Sacc.) Shoemaker


  • Mst. Selina Momtaz Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh
  • Shamim Shamsi Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh
  • Tapan Kumar Dey Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh



Bipolaris leaf blight, antagonistic fungi, radial growth, wheat


Six wheat rhizosphere fungi (Aspergillus flavus, A. fumigatus, A. niger, Penicillium sp., Trichoderma harzianum and T. viride) were evaluated for their antagonistic potentiality towards sixteen isolates of Bipolaris sorokiniana (Sacc.) Shoemaker under different cultural groups. Colony interaction using duel culture technique, inhibition owing to volatile and non-volatile substances in different concentrations were performed. Among them Trichoderma harzianum showed the best performance (colony interaction 61.69-79.02%, volatile substances 44.14-61.34% and non-volatile culture filtrate 47.28-68.14% at 20% concentration) to control Bipolaris sorokiniana followed by Trichoderma viride, Aspergillus niger, Aspergillus flavus, A. fumigatus and Penicillium sp..


Chowdhury, A.K. Garain, P.K. Mukherjee, S. Dutta, S. and Singh, D.P. 2005. Zonate eyespot of wheat. A new report. J. Mycopathological Research. 43 (1), 139-140.

Alam, K.B. Malaker, P.K. Shaheed, M.A. Ahmed, M.U. Ahmed, F. and Haque, M.S. 1995. Yield loss assessment of wheat due to Bipolaris leaf blight in Bangladesh. Bangladesh J. Pl. Pathol. 11 (1&2), 35-38.

Prescott, J.M. Burnett, P.A. Saari, E.E. Ransom, J. Bowman, J. Milliano, Wde. Singh, R.P. and Bekele, G. 1986. Wheat Diseases and Pests: A Guide for Field Identification. CIMMYT, Mexico. 135 pp.

Saari, E.E. 1998. Leaf blight diseases and associated soil borne fungal pathogens of wheat in South and Southeast Asia. In: Helminthosporium Blight of Wheat: Spot Blotch and Tan Spot. Duveiller, E. Dubin, H.J. Reeves, J. and McNab, A. (Eds), CIMMYT, Mexico DF. pp 37-51.

Hollomon, D.W. 2015b. Fungicide Resistance: Facing the Challenge. Plant Protect. Sci. 51, 70-176.

Baker, K.F. and Cook, R.J. 1983. The Nature and Practice of Biological Control of Plant Pathogens. St. Paul, MN, USA: American Phytopathological Society.

Wu, W.S. 1977. Antibiotic and mycoparasitic effects of several fungi against seed and soilborne pathogens associated with wheat and oats. Botanical Bulletin of Academia Sinica, 18, 25-31.

Ole Becker, J. And Schwinn, F.J. 1993. Control of soil-borne pathogens with living bacteria and fungi: status and outlook. Pesticide Science. 37, 355-363.

CAB (Commonwealth Agricultural Bureau) 1968. Plant Pathologist’s Pocket Book. 1st edition. The Commonwealth Mycological Institute, England. 267 pp.

Koch, R. 1883. Uber die neuen Untersuchungsmethodenzum Nachweis der Mikrokosmen in Boden, Luft und Wasser. Vortrag auf dem XI. Deutschen Arztetag in Berlin, Vereinsblatt fur Deutschland, Komnussions-Verlag von F.C.W.Vogel, Leipzig (1883), pp.137, 274-137, 284

Skidmore, A.M. and Dickinson, C.H. 1976. Colony interaction and hyphal interference between Septorianodorum and phylloplane fungi. Trans. Brit. Mycol. Soc. 66, 57-64.

Dennis, C. and Webster, J. 1971 a. Antagonistic properties of species groups of Trichoderma. I. Production of non-volatile antibiotics. Trans. Brit. Mycol. Soc. 57: 25-29.

Dennis, C. and Webster, J. 1971 b. Antagonistic properties of species groups of Trichoderma. II. Production of volatile antibiotics. Trans. Brit. Mycol. Soc. 57: 41-48.

Dennis, C. and Webster, J. 1971 c. Antagonistic properties of species groups of Trichoderma. III. Hyphal interactions. Trans. Brit. Mycol. Soc. 57: 363-369.

Bashar, M.A. and Rai, B. 1994. Antagonistic potential of root region microflora of chickpea against Fusarium oxysporum f. sp. ciceri. Bangladesh J. Bot. 23(1), 13-19.

Prince, L. Raja, A. and Prabakaran, P. 2011. Antagonistic potentiality of some soil mycoflora against Colletotrichum falcatum. World J. Sci. Technol. 1(4), 39-42.

Chowdhury P. 2020. Pathogenic mycoflora of rice and its management. Ph.D. Thesis. Department of Botany, University of Dhaka, Dhaka, Bangladesh.

Yassin, M.T., Mostafa, A.A.F. and Al-Asker, A.A. 2022. In vitro antagonistic activity of Trichoderma spp. against fungal pathogens causing black point disease of wheat. J. of Taibah U. of Sci. 16(1), 57-65.

Salehpour, M. Etebarian, H.R. Roustaei, A. Khodakaramian, G. and Aminian, H. 2005. Biological Control of Common Root Rot of Wheat (Bipolaris sorokiniana) by Trichoderma Isolates. J. Plant Pathol. 4, 85-90.

Prasad, K.S.K., Kulkarni, S. And Siddrmaiah, A.L. 1978. Antagonistic action of Trichoderma spp. and Streptomyces spp. on Drechslera sativum (Helminthosporium sativum Pam King & Bakke) Subram. & Jain. Curro Res. 12, 202-203.

Khan, R.A.A., Najeeb, S., Hussain, S. 2020. Bioactive secondary metabolites from Trichoderma spp. against phytopathogenic fungi. Microorganisms. 8(6), 817.

Akter, M.T. Hossain, K.S. and Bashar, M.A. 2014. Antagonistic potentiality of rhizosphere fungi against leaf spot and fruit rot pathogens of brinjal. Bangladesh J. Bot. 43(2), 213-217.

Yasmin, Z. and Shamsi, S. 2019. Antagonistic potential of soil fungi against Colletotrichum gloeosporiodes (Penz.) Sacc., the causal agent of anthracnose of Rauwolfia serpentine (L.) Benth. Ex Kurz. Dhaka Univ. J. Biol. Sci. 28(2), 219-226.

Bilai, VI 1966. Volatile antibiotics in fungi of the genus Trichoderma. Microbiologia. 25, 458-465.

Dick, C.M. and Hutchinson, S.A. 1966. Biological activity of volatile fungal metabolites. Nature.211, 868.

Faull, J.L. Craene-Cook, K.A. and Pilkington, B. 1994. Production of an isonitrin antibiotic by a UV induced mutant of Trichoderma harzianum. Phytochemistry. 36, 1273-1276.

Lee, C.H. Chang, M.C. Lee, H.J. Kho, Y.H. and Koshiso, H.1995. MR-93A, A new oxazole from Trichoderma harzianum KCTC 0114BP. J. Natl. Prod. 58, 1605-1607.

Lee, C.H. Koshino, H. Chang, M.C. Lee, H.J. and Kho, Y.H. 1995. MR-304A, a new melanin synthesis inhibitor from the fungus Trichoderma harzianum. J. Antibiot. 48, 1168-1170.

Godtfredsen, W.O. and Vangedal, S. 1965. Trichodermin a new sequiterpene antibiotic. Acta Chem. Scand. 19, 1088-1102.

Grove, J.F. Moffatt, J.S. and Vischer, E.B. 1965. Viridin. Part I. Isolation and characterization. J. Chem. Soc.1965, 3803-3811.

Harris, G.H. Jones, E.T.T. Meinz, M.S. Nallin-Omstead, M. and Bills, G.L. 1993. Isolation and structure elucidation of viridiofungins A, B and C. Tetrahedron Lett.34, 5235-5238.

Kumeda, Y. Asao, T. Lida, A. Wada, S. Futami, S. and Fuijita, T. 1994. Effects of ergokonin produced by Trichoderma viride on the growth and morphological development of fungi. Bokin Bobai. 22, 663-670.

Brian, P.W. McGowan, J.C. 1945.Viridin: a highly fungistatic substance produced by Trichoderma viride. Nature 156, 144-145.

Gottlieb, D. and Shaw, P.D. 1970. Mechanism of action of antifungal antibiotics. Ann. Rev. Phytopathol. 8, 371-402.

Krivoshchekova, T.G. and Mishchenk, V.S. 1990. The effectiveness of trichodermin. Zashchita Rastenii (Moskova).11, 22.




How to Cite

Momtaz, M. S., Shamsi, S., & Dey, T. K. (2022). Antagonistic potentiality of six wheat rhizosphere fungi against leaf blight pathogen Bipolaris sorokiniana (Sacc.) Shoemaker. Bioresearch Communications - (BRC), 8(2), 1124–1131.



Original Article