High-Risk Non-synonymous SNPs of Human Bcl-2 Gene Alters Structural Stability and Small Molecule Binding

Authors

  • A. S. M. Zisanur Rahman Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka- 1229, Bangladesh
  • Arittra Bhattacharjee Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka- 1229, Bangladesh
  • Abdullah All Jaber Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka- 1229, Bangladesh
  • Maqsud Hossain Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka- 1229, Bangladesh
  • Kazi Nadim Hasan Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka- 1229, Bangladesh
  • Sohidul Islam Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka- 1229, Bangladesh
  • Zaied Ahmed Bhuyan Department of Biochemistry & Microbiology, North South University, Bashundhara, Dhaka- 1229, Bangladesh

Keywords:

Bcl-2 gene, apoptosis, non-synonymous SNPs, HA 14-1

Abstract

B-cell lymphoma 2 (Bcl-2) gene, which encodes Bcl-2 protein, is vital for apoptotic programmed cell death. In this study, computational approaches were performed to reveal the effect of non-synonymous single nucleotide polymorphisms (nsSNPs) in Bcl-2 gene. A total of 79 nsSNPs were studied. Amongst all, 11 nsSNPs (M166T, G141E, R129C, S105P, F104S, R98L, L97P, H94P, V93A, G27S, V15L) were located at highly conserved region or functionally important domains with a high probability of being deleterious for Bcl-2 structure or role. Except for H94P, rest of the high-risk nsSNPs displayed decreased stability of BcL-2 structure. However, F104S attracted significant attention because it exhibited considerably reduced binding affinity than wild type Bcl-2 protein against HA 14-1 antagonist. This extensive computational experiment will assist as a valuable data source for upcoming population-based studies

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Published

01-01-2020

How to Cite

Rahman, A. S. M. Z., Bhattacharjee, A., Jaber, A. A., Hossain, M., Hasan, K. N. ., Islam, S., & Bhuyan, Z. A. (2020). High-Risk Non-synonymous SNPs of Human Bcl-2 Gene Alters Structural Stability and Small Molecule Binding. Bioresearch Communications - (BRC), 6(1), 791–800. Retrieved from https://www.bioresearchcommunications.com/index.php/brc/article/view/41

Issue

Vol. 6 No. 1 (2020)

Section

Original Article

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