Resistance profiles of Azithromycin in Escherichia coli isolated from human urine

Authors

  • Shaikh Mirja Nurunnabi Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Belal Ahmad Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Joyonta Sutradhar Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Farjana Begum Topa Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Abu Noman Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Arifur Rahman Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Rashid Taqui Sakib Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Nishat Tasnim Nousin Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Shamim Mahmud Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Sadia Sinty Disha Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Ashik Imran Shahjalal University of Science and Technology, Dept. of Biochemistry and Molecular Biology, Sylhet-3114, Bangladesh
  • Mohammed Shahjahan Rajshahi University, Dept. of Biochemistry and Molecular Biology, Rajshahi-6205, Bangladesh

Keywords:

Resistance profile, Azithromycin, E.coli, Urine sample, Resistant plasmid

Abstract

Bacterial drug resistance is now a main hindrance to use antibiotics in treating infectious diseases and global public health concern contributing to increased mortality in low-income countries. The plasmid mediated drug resistance which confers resistant to a number of antibiotics. Here, we studied the azithromycin susceptibility by paper disc plate technique and agar diffusion assay in bacterial strains collected from urine at the department of Microbiology Rajshahi Medical College Hospital, Rajshahi. We found that azithromycin at certain concentration is resistant to Escherichia coli (E. coli) but not Klebsiella, Proteus, Streptococcus and Pseudomonus strains. Furthermore, E. coli was also resistant to number of commonly used antibiotics like Ampicillin, Amoxicillin, Tetracycline, Doxycycline, Ciprofloxacin, Cephradine, PenicillinG and Chloramphenicol. In present study plasmid DNA was isolated from azithromycin resistant E .coli. Together, our primary results suggest that E. coli is multidrug resistant due to possibly the presence of approximately 4kb in size of resistant plasmid in Rajshahi area.

Downloads

Published

01-07-2019

How to Cite

Nurunnabi, S. M., Ahmad, B., Sutradhar, J., Topa, F. B., Noman, A., Rahman, A., Sakib, R. T., Nousin, N. T., Mahmud, S., Disha, S. S., Imran, A., & Shahjahan, M. (2019). Resistance profiles of Azithromycin in Escherichia coli isolated from human urine. Bioresearch Communications - (BRC), 5(2), 707–714. Retrieved from https://www.bioresearchcommunications.com/index.php/brc/article/view/56

Issue

Vol. 5 No. 2 (2019)

Section

Original Article

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Author(s) will retain the copyright of their own articles. By submitting the article to Bioresearch Communications (BRC), the author(s) have granted the BRC for the use of the article.